Device

Part:BBa_K1058008:Design

Designed by: Tian A'min   Group: iGEM13_BIT   (2013-08-31)


Cr(VI) sensor with egfp(PchrB+chrB+egfp)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 135
    Illegal BamHI site found at 1109
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We'd like to design an integrated sensor with an amplifier and controller for milk product. Cr(VI) can sometimes exist in milk because of the illegal adding, so a part sensitive to Cr(VI) is involved in our consideration.


Source

Ochrobactrum tritici

References

Branco R, Alpoim MC, Morais PV. (2004) Ochrobactrum tritici strain 5bvl1 - characterization of a Cr(VI)-resistant and Cr(VI)-reducing strain. Can J Microbiol 50(9): 697-703.

Branco R, Morais PV. (2013) Identification and characterization of the transcriptional regulator ChrB in the chromate resistance determinant of Ochrobactrum tritici 5bvl1. PLoS ONE 8: 77987.

Branco R, Chung AP, Johnston T, Gurel V, Morais P, Zhitkovich A. (2008) The chromate-inducible chrBACF operon from the transposable element TnOtChr confers resistance to chromium(VI) and superoxide. J Bacteriol 190(21): 6996-7003.

Branco R, Cristovao A, Morais PV. (2013) Highly sensitive, highly specific whole-cell bioreporters for the detection of chromate in environmental samples. PLoS ONE 8(1): e54005.